The LDH Leakage assay determines the membrane integrity/extent of LDH leakage from cells with a damaged membrane under the influence of test item. The assay is a fluorometric measure of the release of lactate dehydrogenase (LDH) from cells with a damaged membrane by conversion of resazurin into resorufin that is measured by change in absorbance. This assay can be performed with HepG2 or L-929 cells.

Multiple cell types may be used, depending on the pharmacological action, potential target, metabolism and mechanism, or end point of the agent.

Types of cells available: